LncRNA NR2F1-AS1 inhibits the malignant properties of cervical cancer cells via targeting miR-642a-3p/NR2F1 axis

LncRNA NR2F1-AS1 inhibits the malignant properties of cervical cancer cells via targeting miR-642a-3p/NR2F1 axis

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Background: Cervical cancer (CC), as a serious menace to the health of women, has long been one of the most lethal gynecologic neoplasms throughout the world.Long simply southern cat shirt non-coding RNA (LncRNA) NR2F1-AS1 has been documented to exert crucial functions in many malignant tumors.Nonetheless, the function and molecular mechanism of NR2F1-AS1 in CC remain completely unknown.Objective: This study aimed to explore the function and molecular mechanism of NR2F1-AS1 in CC.

Methods: The expression levels of NR2F1-AS1, miR-642a-3p, NR2F1 in CC tissues, and cell lines were examined by reverse transcription real-time quantitative polymerase chain reaction.Cell viability, proliferation, migration, and invasion were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, colony formation and Transwell assays.The protein d2 fleshripper levels of epithelial-mesenchymal transition markers and NR2F1 in CC cells were assessed by Western blot analysis.The correlations among NR2F1-AS1, miR-642a-3p, and NR2F1 were estimated through luciferase reporter and RNA immunoprecipitation assays.

Results: NR2F1-AS1 expression was clearly downregulated in CC tissues and cell lines.Molecular mechanistic experiments showed that NR2F1-AS1 overexpression upregulated NR2F1 expression in CC cells by directly binding to miR-642a-3p, and inhibiting by this way cell viability, proliferation, migration, and invasion in CC.Rescue assays showed that NR2F1 knockdown or miR-642a-3p overexpression offset NR2F1-AS1 upregulation-induced inhibition on CC cell malignant phenotypes.Conclusion: These findings revealed that NR2F1-AS1 played a tumor suppressor role in CC by mediating the miR-642a-3p/NR2F1 axis.